Enum: CellFreeExpressionSystemEnum
Source extracts and reconstituted systems used for cell-free protein synthesis. Crude lysate systems are derived from cell extracts, while reconstituted systems (e.g. PURE) are assembled from purified components.
URI: valuesets:CellFreeExpressionSystemEnum
Permissible Values
| Value | Meaning | Description | Broad Mappings | Advantage | Domain | Extract Type | Source Taxon |
|---|---|---|---|---|---|---|---|
| ECOLI_EXTRACT | Escherichia coli crude cell extract (e.g. S30, S12), the most widely used prokaryotic cell-free system for high-yield protein production | BAO:0000366 | prokaryotic | crude lysate | NCBITaxon:562 | ||
| PURE_SYSTEM | Protein synthesis Using Recombinant Elements - a reconstituted system assembled from individually purified E. coli translation factors, ribosomes, and enzymes | BAO:0000366 | defined composition, low nuclease/protease activity | prokaryotic | reconstituted | ||
| WHEAT_GERM_EXTRACT | Wheat germ (Triticum aestivum) extract, a eukaryotic system favored for expression of complex and difficult eukaryotic proteins | BAO:0000366 | eukaryotic | crude lysate | NCBITaxon:4565 | ||
| RABBIT_RETICULOCYTE_LYSATE | BAO:0000255 | Rabbit (Oryctolagus cuniculus) reticulocyte lysate, a classic mammalian system for in vitro translation and protein labeling | eukaryotic | crude lysate | NCBITaxon:9986 | ||
| INSECT_CELL_EXTRACT | Insect cell extract, typically from Spodoptera frugiperda (Sf21), supporting eukaryotic post-translational modifications | BAO:0000366 | eukaryotic | crude lysate | NCBITaxon:7108 | ||
| HELA_EXTRACT | Human HeLa cell extract used for mammalian cell-free expression with authentic human translation machinery | BAO:0000366 | eukaryotic | crude lysate | NCBITaxon:9606 | ||
| CHO_EXTRACT | Chinese hamster ovary (Cricetulus griseus) cell extract supporting glycosylation and disulfide bond formation | BAO:0000366 | eukaryotic | crude lysate | NCBITaxon:10029 | ||
| YEAST_EXTRACT | Yeast (Saccharomyces cerevisiae) cell extract for eukaryotic cell-free protein synthesis | BAO:0000366 | eukaryotic | crude lysate | NCBITaxon:4932 | ||
| LEISHMANIA_EXTRACT | Leishmania tarentolae cell extract, a eukaryotic system supporting high yields and post-translational modifications | BAO:0000366 | eukaryotic | crude lysate | NCBITaxon:5689 | ||
| VIBRIO_NATRIEGENS_EXTRACT | Vibrio natriegens cell extract, an emerging fast-growing prokaryotic chassis for rapid, high-yield cell-free expression | BAO:0000366 | prokaryotic | crude lysate | NCBITaxon:691 | ||
| TOBACCO_BY2_EXTRACT | Tobacco BY-2 (Nicotiana tabacum bright yellow-2) cell lysate, a plant eukaryotic cell-free system | BAO:0000366 | eukaryotic | crude lysate |
Slots
| Name | Description |
|---|---|
| cell_free_system | The source extract or reconstituted system used for cell-free expression |
See Also
Identifier and Mapping Information
Schema Source
- from schema: https://w3id.org/valuesets
LinkML Source
name: CellFreeExpressionSystemEnum
instantiates:
- valuesets_meta:ValueSetEnumDefinition
description: Source extracts and reconstituted systems used for cell-free protein
synthesis. Crude lysate systems are derived from cell extracts, while reconstituted
systems (e.g. PURE) are assembled from purified components.
title: Cell-Free Expression Systems
from_schema: https://w3id.org/valuesets
see_also:
- https://en.wikipedia.org/wiki/Cell-free_protein_synthesis
contributors:
- orcid:0000-0002-6601-2165
- https://github.com/anthropics/claude-code
status: DRAFT
rank: 1000
permissible_values:
ECOLI_EXTRACT:
text: ECOLI_EXTRACT
description: Escherichia coli crude cell extract (e.g. S30, S12), the most widely
used prokaryotic cell-free system for high-yield protein production
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:562
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: prokaryotic
aliases:
- E. coli extract
- E. coli lysate
- S30 extract
- ECE
broad_mappings:
- BAO:0000366
PURE_SYSTEM:
text: PURE_SYSTEM
description: Protein synthesis Using Recombinant Elements - a reconstituted system
assembled from individually purified E. coli translation factors, ribosomes,
and enzymes
annotations:
extract_type:
tag: extract_type
value: reconstituted
domain:
tag: domain
value: prokaryotic
advantage:
tag: advantage
value: defined composition, low nuclease/protease activity
aliases:
- PURE
- PURExpress
- reconstituted system
broad_mappings:
- BAO:0000366
WHEAT_GERM_EXTRACT:
text: WHEAT_GERM_EXTRACT
description: Wheat germ (Triticum aestivum) extract, a eukaryotic system favored
for expression of complex and difficult eukaryotic proteins
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:4565
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: eukaryotic
aliases:
- WGE
- wheat germ system
broad_mappings:
- BAO:0000366
RABBIT_RETICULOCYTE_LYSATE:
text: RABBIT_RETICULOCYTE_LYSATE
description: Rabbit (Oryctolagus cuniculus) reticulocyte lysate, a classic mammalian
system for in vitro translation and protein labeling
meaning: BAO:0000255
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:9986
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: eukaryotic
aliases:
- RRL
- reticulocyte lysate
- rabbit reticulocyte lysate format
INSECT_CELL_EXTRACT:
text: INSECT_CELL_EXTRACT
description: Insect cell extract, typically from Spodoptera frugiperda (Sf21),
supporting eukaryotic post-translational modifications
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:7108
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: eukaryotic
aliases:
- ICE
- Sf21 extract
- insect lysate
broad_mappings:
- BAO:0000366
HELA_EXTRACT:
text: HELA_EXTRACT
description: Human HeLa cell extract used for mammalian cell-free expression with
authentic human translation machinery
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:9606
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: eukaryotic
aliases:
- HeLa lysate
- human cell extract
broad_mappings:
- BAO:0000366
CHO_EXTRACT:
text: CHO_EXTRACT
description: Chinese hamster ovary (Cricetulus griseus) cell extract supporting
glycosylation and disulfide bond formation
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:10029
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: eukaryotic
aliases:
- CHO lysate
broad_mappings:
- BAO:0000366
YEAST_EXTRACT:
text: YEAST_EXTRACT
description: Yeast (Saccharomyces cerevisiae) cell extract for eukaryotic cell-free
protein synthesis
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:4932
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: eukaryotic
aliases:
- S. cerevisiae extract
- yeast lysate
broad_mappings:
- BAO:0000366
LEISHMANIA_EXTRACT:
text: LEISHMANIA_EXTRACT
description: Leishmania tarentolae cell extract, a eukaryotic system supporting
high yields and post-translational modifications
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:5689
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: eukaryotic
aliases:
- Leishmania tarentolae extract
- LTE
broad_mappings:
- BAO:0000366
VIBRIO_NATRIEGENS_EXTRACT:
text: VIBRIO_NATRIEGENS_EXTRACT
description: Vibrio natriegens cell extract, an emerging fast-growing prokaryotic
chassis for rapid, high-yield cell-free expression
annotations:
source_taxon:
tag: source_taxon
value: NCBITaxon:691
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: prokaryotic
aliases:
- V. natriegens extract
- Vmax extract
broad_mappings:
- BAO:0000366
TOBACCO_BY2_EXTRACT:
text: TOBACCO_BY2_EXTRACT
description: Tobacco BY-2 (Nicotiana tabacum bright yellow-2) cell lysate, a plant
eukaryotic cell-free system
annotations:
extract_type:
tag: extract_type
value: crude lysate
domain:
tag: domain
value: eukaryotic
aliases:
- BY-2 lysate
- BYL
broad_mappings:
- BAO:0000366