Enum: CellFreeReactionFormatEnum

Physical configurations of cell-free expression reactions, differing in how substrates are supplied and byproducts removed, which affects reaction duration and protein yield.

URI: valuesets:CellFreeReactionFormatEnum

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Permissible Values

Value	Meaning	Description	Related Mappings	Advantage	Duration
BATCH		Single closed reaction with all components mixed at the start; simplest format but limited by substrate depletion and byproduct accumulation			short (hours)
CONTINUOUS_EXCHANGE		Continuous-exchange cell-free (CECF) format with a semipermeable membrane separating a reaction chamber from a feeding chamber, allowing diffusion of substrates in and byproducts out	OBI:0600052		extended (up to ~24 h)
CONTINUOUS_FLOW		Continuous-flow cell-free (CFCF) format in which fresh substrates are continuously pumped through the reaction while product is collected			extended
BILAYER		Bilayer format where a feeding solution is layered over the reaction mixture without a membrane, prolonging reaction by passive diffusion
LYOPHILIZED		Freeze-dried (lyophilized) cell-free reaction that is shelf-stable and activated by rehydration; basis of freeze-dried cell-free (FD-CF) diagnostics and portable biomanufacturing	NCIT:C28150	cold-chain-free storage and distribution

Slots

Name	Description
cell_free_reaction_format	The physical configuration of the cell-free reaction

Identifier and Mapping Information

Schema Source

• from schema: https://w3id.org/valuesets

LinkML Source

name: CellFreeReactionFormatEnum
instantiates:
- valuesets_meta:ValueSetEnumDefinition
description: Physical configurations of cell-free expression reactions, differing
  in how substrates are supplied and byproducts removed, which affects reaction duration
  and protein yield.
title: Cell-Free Reaction Formats
from_schema: https://w3id.org/valuesets
contributors:
- orcid:0000-0002-6601-2165
- https://github.com/anthropics/claude-code
status: DRAFT
rank: 1000
permissible_values:
  BATCH:
    text: BATCH
    description: Single closed reaction with all components mixed at the start; simplest
      format but limited by substrate depletion and byproduct accumulation
    annotations:
      duration:
        tag: duration
        value: short (hours)
    aliases:
    - batch mode
  CONTINUOUS_EXCHANGE:
    text: CONTINUOUS_EXCHANGE
    description: Continuous-exchange cell-free (CECF) format with a semipermeable
      membrane separating a reaction chamber from a feeding chamber, allowing diffusion
      of substrates in and byproducts out
    annotations:
      duration:
        tag: duration
        value: extended (up to ~24 h)
    aliases:
    - CECF
    - continuous exchange
    - dialysis mode
    related_mappings:
    - OBI:0600052
  CONTINUOUS_FLOW:
    text: CONTINUOUS_FLOW
    description: Continuous-flow cell-free (CFCF) format in which fresh substrates
      are continuously pumped through the reaction while product is collected
    annotations:
      duration:
        tag: duration
        value: extended
    aliases:
    - CFCF
    - continuous flow
  BILAYER:
    text: BILAYER
    description: Bilayer format where a feeding solution is layered over the reaction
      mixture without a membrane, prolonging reaction by passive diffusion
    aliases:
    - bilayer mode
  LYOPHILIZED:
    text: LYOPHILIZED
    description: Freeze-dried (lyophilized) cell-free reaction that is shelf-stable
      and activated by rehydration; basis of freeze-dried cell-free (FD-CF) diagnostics
      and portable biomanufacturing
    annotations:
      advantage:
        tag: advantage
        value: cold-chain-free storage and distribution
    aliases:
    - freeze-dried
    - FD-CF
    - lyophilised
    related_mappings:
    - NCIT:C28150