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Enum: EnergyRegenerationSubstrateEnum

Secondary energy substrates added to cell-free reactions to regenerate ATP and GTP consumed during transcription and translation. Choice of substrate affects cost, reaction longevity, and inorganic phosphate accumulation.

URI: valuesets:EnergyRegenerationSubstrateEnum

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Permissible Values

Value Meaning Description Enzyme
PHOSPHOCREATINE CHEBI:17287 Creatine phosphate (N-phosphocreatine) regenerated with creatine kinase; a classic high-energy phosphate donor creatine kinase
PHOSPHOENOLPYRUVATE CHEBI:18021 Phosphoenolpyruvate (PEP) regenerated with pyruvate kinase; a common high-energy phosphate donor pyruvate kinase
ACETYL_PHOSPHATE CHEBI:15350 Acetyl phosphate regenerated with acetate kinase; an inexpensive energy source acetate kinase
THREE_PGA CHEBI:17794 3-phosphoglycerate (3-PGA), a glycolytic intermediate widely used as a low-cost energy substrate in E. coli cell-free systems
GLUCOSE CHEBI:17634 Glucose feeding glycolysis for ATP regeneration; a very low-cost energy source that requires phosphate buffering
GLUCOSE_6_PHOSPHATE CHEBI:14314 Glucose 6-phosphate fed into glycolysis for energy regeneration
MALTOSE CHEBI:17306 Maltose used as a low-cost energy substrate that limits inorganic phosphate accumulation
PYRUVATE CHEBI:15361 Pyruvate used as an energy source, oxidized via the central metabolism of the extract
MALTODEXTRIN Maltodextrin (glucose polymer) energy substrate providing slow glucose release and minimal phosphate buildup

Slots

Name Description
energy_regeneration_substrate Secondary energy substrate used to regenerate ATP/GTP in the reaction

Identifier and Mapping Information

Schema Source

  • from schema: https://w3id.org/valuesets

LinkML Source

name: EnergyRegenerationSubstrateEnum
instantiates:
- valuesets_meta:ValueSetEnumDefinition
description: Secondary energy substrates added to cell-free reactions to regenerate
  ATP and GTP consumed during transcription and translation. Choice of substrate affects
  cost, reaction longevity, and inorganic phosphate accumulation.
title: Energy Regeneration Substrates
from_schema: https://w3id.org/valuesets
contributors:
- orcid:0000-0002-6601-2165
- https://github.com/anthropics/claude-code
status: DRAFT
rank: 1000
permissible_values:
  PHOSPHOCREATINE:
    text: PHOSPHOCREATINE
    description: Creatine phosphate (N-phosphocreatine) regenerated with creatine
      kinase; a classic high-energy phosphate donor
    meaning: CHEBI:17287
    annotations:
      enzyme:
        tag: enzyme
        value: creatine kinase
    aliases:
    - creatine phosphate
    - phosphocreatine
    - N-phosphocreatine
  PHOSPHOENOLPYRUVATE:
    text: PHOSPHOENOLPYRUVATE
    description: Phosphoenolpyruvate (PEP) regenerated with pyruvate kinase; a common
      high-energy phosphate donor
    meaning: CHEBI:18021
    annotations:
      enzyme:
        tag: enzyme
        value: pyruvate kinase
    aliases:
    - PEP
  ACETYL_PHOSPHATE:
    text: ACETYL_PHOSPHATE
    description: Acetyl phosphate regenerated with acetate kinase; an inexpensive
      energy source
    meaning: CHEBI:15350
    annotations:
      enzyme:
        tag: enzyme
        value: acetate kinase
    aliases:
    - AcP
    - acetyl dihydrogen phosphate
  THREE_PGA:
    text: THREE_PGA
    description: 3-phosphoglycerate (3-PGA), a glycolytic intermediate widely used
      as a low-cost energy substrate in E. coli cell-free systems
    meaning: CHEBI:17794
    aliases:
    - 3-PGA
    - 3-phosphoglycerate
    - 3-phospho-D-glycerate
    - 3-phospho-D-glyceric acid
  GLUCOSE:
    text: GLUCOSE
    description: Glucose feeding glycolysis for ATP regeneration; a very low-cost
      energy source that requires phosphate buffering
    meaning: CHEBI:17634
    aliases:
    - D-glucose
  GLUCOSE_6_PHOSPHATE:
    text: GLUCOSE_6_PHOSPHATE
    description: Glucose 6-phosphate fed into glycolysis for energy regeneration
    meaning: CHEBI:14314
    aliases:
    - G6P
    - glucose-6-phosphate
    - D-glucose 6-phosphate
  MALTOSE:
    text: MALTOSE
    description: Maltose used as a low-cost energy substrate that limits inorganic
      phosphate accumulation
    meaning: CHEBI:17306
  PYRUVATE:
    text: PYRUVATE
    description: Pyruvate used as an energy source, oxidized via the central metabolism
      of the extract
    meaning: CHEBI:15361
  MALTODEXTRIN:
    text: MALTODEXTRIN
    description: Maltodextrin (glucose polymer) energy substrate providing slow glucose
      release and minimal phosphate buildup
    aliases:
    - polymeric glucose