Enum: EnzymologyAssayEnum

Assays for characterizing enzyme function including activity, kinetics, inhibition, and substrate specificity.

Permissible Values

Value	Meaning	Description	Companion Enum Enzyme	Companion Enum Readout	Companion Enum Substrate
ENZYME_ACTIVITY_ASSAY	BAO:0002994	Measures the effect of a perturbagen on enzyme activity	Protein	DetectionModeEnum	ChemicalEntity
MICHAELIS_MENTEN_KINETICS		Enzyme kinetics assay measuring Km and Vmax parameters	Protein		ChemicalEntity
INHIBITION_ASSAY		Assay to measure enzyme inhibition (IC50, Ki)	Protein		ChemicalEntity
SUBSTRATE_SPECIFICITY		Assay to determine enzyme substrate preference and specificity	Protein		ChemicalEntity
FLUOROMETRIC_ENZYME_ASSAY		Enzyme assay using fluorescent substrates or products for detection	Protein	DetectionModeEnum	ChemicalEntity
COLORIMETRIC_ENZYME_ASSAY		Enzyme assay using chromogenic substrates for absorbance-based detection	Protein	DetectionModeEnum	ChemicalEntity
COUPLED_ENZYME_ASSAY		Enzyme assay using a secondary enzyme reaction for signal generation	Protein		ChemicalEntity
CONTINUOUS_ENZYME_ASSAY		Real-time monitoring of enzyme reaction progress	Protein		ChemicalEntity
ENDPOINT_ENZYME_ASSAY		Single time-point measurement of enzyme reaction product	Protein		ChemicalEntity

Slots

Name	Description
enzymology_assay	Enzymology assay type

Identifier and Mapping Information

Schema Source

from schema: https://w3id.org/valuesets

LinkML Source

name: EnzymologyAssayEnum
instantiates:
- valuesets_meta:ValueSetEnumDefinition
description: Assays for characterizing enzyme function including activity, kinetics,
  inhibition, and substrate specificity.
title: Enzymology Assay Types
from_schema: https://w3id.org/valuesets
contributors:
- orcid:0000-0002-6601-2165
- https://github.com/anthropics/claude-code
status: DRAFT
rank: 1000
permissible_values:
  ENZYME_ACTIVITY_ASSAY:
    text: ENZYME_ACTIVITY_ASSAY
    description: Measures the effect of a perturbagen on enzyme activity
    meaning: BAO:0002994
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein
      companion_enum_readout:
        tag: companion_enum_readout
        value: DetectionModeEnum
  MICHAELIS_MENTEN_KINETICS:
    text: MICHAELIS_MENTEN_KINETICS
    description: Enzyme kinetics assay measuring Km and Vmax parameters
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein
  INHIBITION_ASSAY:
    text: INHIBITION_ASSAY
    description: Assay to measure enzyme inhibition (IC50, Ki)
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein
  SUBSTRATE_SPECIFICITY:
    text: SUBSTRATE_SPECIFICITY
    description: Assay to determine enzyme substrate preference and specificity
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein
  FLUOROMETRIC_ENZYME_ASSAY:
    text: FLUOROMETRIC_ENZYME_ASSAY
    description: Enzyme assay using fluorescent substrates or products for detection
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein
      companion_enum_readout:
        tag: companion_enum_readout
        value: DetectionModeEnum
  COLORIMETRIC_ENZYME_ASSAY:
    text: COLORIMETRIC_ENZYME_ASSAY
    description: Enzyme assay using chromogenic substrates for absorbance-based detection
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein
      companion_enum_readout:
        tag: companion_enum_readout
        value: DetectionModeEnum
  COUPLED_ENZYME_ASSAY:
    text: COUPLED_ENZYME_ASSAY
    description: Enzyme assay using a secondary enzyme reaction for signal generation
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein
  CONTINUOUS_ENZYME_ASSAY:
    text: CONTINUOUS_ENZYME_ASSAY
    description: Real-time monitoring of enzyme reaction progress
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein
  ENDPOINT_ENZYME_ASSAY:
    text: ENDPOINT_ENZYME_ASSAY
    description: Single time-point measurement of enzyme reaction product
    annotations:
      companion_enum_substrate:
        tag: companion_enum_substrate
        value: ChemicalEntity
      companion_enum_enzyme:
        tag: companion_enum_enzyme
        value: Protein