Enum: GOEvidenceCode

Gene Ontology evidence codes used to describe the type of support for GO annotations. Each code maps to the Evidence and Conclusion Ontology (ECO). Evidence codes are categorized by whether they represent experimental evidence, computational analysis, phylogenetic inference, author statements, or electronic annotation. All codes except IEA represent manual curation.

URI: valuesets:GOEvidenceCode

Permissible Values

Value	Title	Meaning	Description	Is Author Statement	Is Computational	Is Curator Inference	Is Electronic	Is Experimental	Is High Throughput	Is Manual	Is Phylogenetic
EXP	Inferred from Experiment	ECO:0000269	General experimental evidence supporting a GO annotation					True		True
IDA	Inferred from Direct Assay	ECO:0000314	Evidence from a direct assay for the presence or activity of a gene product					True		True
IPI	Inferred from Physical Interaction	ECO:0000353	Evidence from physical interaction between gene products					True		True
IMP	Inferred from Mutant Phenotype	ECO:0000315	Evidence from the phenotype of a mutant allele					True		True
IGI	Inferred from Genetic Interaction	ECO:0000316	Evidence from genetic interaction between genes					True		True
IEP	Inferred from Expression Pattern	ECO:0000270	Evidence from gene expression pattern data					True		True
HTP	Inferred from High Throughput Experiment	ECO:0006056	Evidence from high-throughput experimental methods					True	True	True
HDA	Inferred from High Throughput Direct Assay	ECO:0007005	Evidence from high-throughput direct assay experiments					True	True	True
HMP	Inferred from High Throughput Mutant Phenotype	ECO:0007001	Evidence from high-throughput mutant phenotype screening					True	True	True
HGI	Inferred from High Throughput Genetic Interaction	ECO:0007003	Evidence from high-throughput genetic interaction screening					True	True	True
HEP	Inferred from High Throughput Expression Pattern	ECO:0007007	Evidence from high-throughput expression profiling					True	True	True
IBA	Inferred from Biological aspect of Ancestor	ECO:0000318	Evidence from conservation of biological aspect in an ancestral sequence							True	True
IBD	Inferred from Biological aspect of Descendant	ECO:0000319	Evidence from conservation of biological aspect in a descendant sequence							True	True
IKR	Inferred from Key Residues	ECO:0000320	Evidence from phylogenetic analysis showing loss of key residues							True	True
IRD	Inferred from Rapid Divergence	ECO:0000321	Evidence from rapid divergence from ancestral sequence							True	True
ISS	Inferred from Sequence or structural Similarity	ECO:0000250	Evidence from sequence or structural similarity to another annotated gene product		True					True
ISO	Inferred from Sequence Orthology	ECO:0000266	Evidence from orthology to an experimentally characterized gene product		True					True
ISA	Inferred from Sequence Alignment	ECO:0000247	Evidence from sequence alignment to an annotated gene product		True					True
ISM	Inferred from Sequence Model	ECO:0000255	Evidence from match to a sequence model (e.g., HMM, profile)		True					True
IGC	Inferred from Genomic Context	ECO:0000317	Evidence from genomic context such as synteny or gene neighborhood		True					True
RCA	Inferred from Reviewed Computational Analysis	ECO:0000245	Evidence from computational analysis that has been reviewed by a curator		True					True
TAS	Traceable Author Statement	ECO:0000304	Evidence from an author statement in a published paper that can be traced to the original source	True						True
NAS	Non-traceable Author Statement	ECO:0000303	Evidence from an author statement that cannot be traced to the experimental source	True						True
IC	Inferred by Curator	ECO:0000305	Evidence inferred by a curator based on existing annotations and biological knowledge			True				True
ND	No biological Data available	ECO:0000307	Indicates that no biological data is available to support an annotation							True
IEA	Inferred from Electronic Annotation	ECO:0000501	Evidence from automated electronic annotation pipelines without manual curation				True

Slots

Name	Description
go_evidence	Gene Ontology evidence codes mapped to Evidence and Conclusion Ontology (ECO)...

Identifier and Mapping Information

Annotations

property	value
stewards	https://geneontology.org
publishers	https://geneontology.org

Schema Source

• from schema: https://w3id.org/linkml/valuesets

LinkML Source

name: GOEvidenceCode
instantiates:
- valuesets_meta:ValueSetEnumDefinition
annotations:
  stewards:
    tag: stewards
    value: https://geneontology.org
  publishers:
    tag: publishers
    value: https://geneontology.org
description: Gene Ontology evidence codes used to describe the type of support for
  GO annotations. Each code maps to the Evidence and Conclusion Ontology (ECO). Evidence
  codes are categorized by whether they represent experimental evidence, computational
  analysis, phylogenetic inference, author statements, or electronic annotation. All
  codes except IEA represent manual curation.
title: GO Evidence Code
from_schema: https://w3id.org/linkml/valuesets
contributors:
- orcid:0000-0002-6601-2165
- https://github.com/anthropics/claude-code
status: STANDARD
rank: 1000
permissible_values:
  EXP:
    text: EXP
    description: General experimental evidence supporting a GO annotation
    meaning: ECO:0000269
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
    title: Inferred from Experiment
    aliases:
    - experimental evidence used in manual assertion
  IDA:
    text: IDA
    description: Evidence from a direct assay for the presence or activity of a gene
      product
    meaning: ECO:0000314
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
    title: Inferred from Direct Assay
    aliases:
    - direct assay evidence used in manual assertion
  IPI:
    text: IPI
    description: Evidence from physical interaction between gene products
    meaning: ECO:0000353
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
    title: Inferred from Physical Interaction
    aliases:
    - physical interaction evidence used in manual assertion
  IMP:
    text: IMP
    description: Evidence from the phenotype of a mutant allele
    meaning: ECO:0000315
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
    title: Inferred from Mutant Phenotype
    aliases:
    - mutant phenotype evidence used in manual assertion
  IGI:
    text: IGI
    description: Evidence from genetic interaction between genes
    meaning: ECO:0000316
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
    title: Inferred from Genetic Interaction
    aliases:
    - genetic interaction evidence used in manual assertion
  IEP:
    text: IEP
    description: Evidence from gene expression pattern data
    meaning: ECO:0000270
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
    title: Inferred from Expression Pattern
    aliases:
    - expression pattern evidence used in manual assertion
  HTP:
    text: HTP
    description: Evidence from high-throughput experimental methods
    meaning: ECO:0006056
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: true
    title: Inferred from High Throughput Experiment
    aliases:
    - high throughput evidence used in manual assertion
  HDA:
    text: HDA
    description: Evidence from high-throughput direct assay experiments
    meaning: ECO:0007005
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: true
    title: Inferred from High Throughput Direct Assay
    aliases:
    - high throughput direct assay evidence used in manual assertion
  HMP:
    text: HMP
    description: Evidence from high-throughput mutant phenotype screening
    meaning: ECO:0007001
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: true
    title: Inferred from High Throughput Mutant Phenotype
    aliases:
    - high throughput mutant phenotypic evidence used in manual assertion
  HGI:
    text: HGI
    description: Evidence from high-throughput genetic interaction screening
    meaning: ECO:0007003
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: true
    title: Inferred from High Throughput Genetic Interaction
    aliases:
    - high throughput genetic interaction phenotypic evidence used in manual assertion
  HEP:
    text: HEP
    description: Evidence from high-throughput expression profiling
    meaning: ECO:0007007
    annotations:
      is_experimental:
        tag: is_experimental
        value: true
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: true
    title: Inferred from High Throughput Expression Pattern
    aliases:
    - high throughput expression pattern evidence used in manual assertion
  IBA:
    text: IBA
    description: Evidence from conservation of biological aspect in an ancestral sequence
    meaning: ECO:0000318
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_phylogenetic:
        tag: is_phylogenetic
        value: true
    title: Inferred from Biological aspect of Ancestor
    aliases:
    - biological aspect of ancestor evidence used in manual assertion
  IBD:
    text: IBD
    description: Evidence from conservation of biological aspect in a descendant sequence
    meaning: ECO:0000319
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_phylogenetic:
        tag: is_phylogenetic
        value: true
    title: Inferred from Biological aspect of Descendant
    aliases:
    - biological aspect of descendant evidence used in manual assertion
  IKR:
    text: IKR
    description: Evidence from phylogenetic analysis showing loss of key residues
    meaning: ECO:0000320
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_phylogenetic:
        tag: is_phylogenetic
        value: true
    title: Inferred from Key Residues
    aliases:
    - phylogenetic determination of loss of key residues evidence used in manual assertion
  IRD:
    text: IRD
    description: Evidence from rapid divergence from ancestral sequence
    meaning: ECO:0000321
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_phylogenetic:
        tag: is_phylogenetic
        value: true
    title: Inferred from Rapid Divergence
    aliases:
    - rapid divergence from ancestral sequence evidence used in manual assertion
  ISS:
    text: ISS
    description: Evidence from sequence or structural similarity to another annotated
      gene product
    meaning: ECO:0000250
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_computational:
        tag: is_computational
        value: true
    title: Inferred from Sequence or structural Similarity
    aliases:
    - sequence similarity evidence used in manual assertion
  ISO:
    text: ISO
    description: Evidence from orthology to an experimentally characterized gene product
    meaning: ECO:0000266
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_computational:
        tag: is_computational
        value: true
    title: Inferred from Sequence Orthology
    aliases:
    - sequence orthology evidence used in manual assertion
  ISA:
    text: ISA
    description: Evidence from sequence alignment to an annotated gene product
    meaning: ECO:0000247
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_computational:
        tag: is_computational
        value: true
    title: Inferred from Sequence Alignment
    aliases:
    - sequence alignment evidence used in manual assertion
  ISM:
    text: ISM
    description: Evidence from match to a sequence model (e.g., HMM, profile)
    meaning: ECO:0000255
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_computational:
        tag: is_computational
        value: true
    title: Inferred from Sequence Model
    aliases:
    - match to sequence model evidence used in manual assertion
  IGC:
    text: IGC
    description: Evidence from genomic context such as synteny or gene neighborhood
    meaning: ECO:0000317
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_computational:
        tag: is_computational
        value: true
    title: Inferred from Genomic Context
    aliases:
    - genomic context evidence used in manual assertion
  RCA:
    text: RCA
    description: Evidence from computational analysis that has been reviewed by a
      curator
    meaning: ECO:0000245
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_computational:
        tag: is_computational
        value: true
    title: Inferred from Reviewed Computational Analysis
    aliases:
    - automatically integrated combinatorial evidence used in manual assertion
  TAS:
    text: TAS
    description: Evidence from an author statement in a published paper that can be
      traced to the original source
    meaning: ECO:0000304
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_author_statement:
        tag: is_author_statement
        value: true
    title: Traceable Author Statement
    aliases:
    - author statement supported by traceable reference used in manual assertion
  NAS:
    text: NAS
    description: Evidence from an author statement that cannot be traced to the experimental
      source
    meaning: ECO:0000303
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_author_statement:
        tag: is_author_statement
        value: true
    title: Non-traceable Author Statement
    aliases:
    - author statement without traceable support used in manual assertion
  IC:
    text: IC
    description: Evidence inferred by a curator based on existing annotations and
      biological knowledge
    meaning: ECO:0000305
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_curator_inference:
        tag: is_curator_inference
        value: true
    title: Inferred by Curator
    aliases:
    - curator inference used in manual assertion
  ND:
    text: ND
    description: Indicates that no biological data is available to support an annotation
    meaning: ECO:0000307
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: true
      is_high_throughput:
        tag: is_high_throughput
        value: false
    title: No biological Data available
    aliases:
    - no evidence data found used in manual assertion
  IEA:
    text: IEA
    description: Evidence from automated electronic annotation pipelines without manual
      curation
    meaning: ECO:0000501
    annotations:
      is_experimental:
        tag: is_experimental
        value: false
      is_manual:
        tag: is_manual
        value: false
      is_high_throughput:
        tag: is_high_throughput
        value: false
      is_electronic:
        tag: is_electronic
        value: true
    title: Inferred from Electronic Annotation
    aliases:
    - evidence used in automatic assertion