Skip to content

Enum: InteractionDetectionMethod

Methods used to detect molecular interactions

URI: valuesets:InteractionDetectionMethod

Permissible Values

Value Meaning Description
TWO_HYBRID MI:0018 Classical two-hybrid system using transcriptional activity
COIMMUNOPRECIPITATION MI:0019 Using antibody to capture bait and its ligands
PULL_DOWN MI:0096 Affinity capture using immobilized bait
TANDEM_AFFINITY_PURIFICATION MI:0676 TAP tagging for protein complex purification
FLUORESCENCE_RESONANCE_ENERGY_TRANSFER MI:0055 FRET for detecting proximity between molecules
SURFACE_PLASMON_RESONANCE MI:0107 SPR for real-time binding analysis
CROSS_LINKING MI:0030 Chemical cross-linking of interacting proteins
X_RAY_CRYSTALLOGRAPHY MI:0114 Crystal structure determination
NMR MI:0077 Nuclear magnetic resonance spectroscopy
ELECTRON_MICROSCOPY MI:0040 EM for structural determination
MASS_SPECTROMETRY MI:0943 MS-based interaction detection
PROXIMITY_LIGATION_ASSAY MI:0813 PLA for detecting protein proximity
BIMOLECULAR_FLUORESCENCE_COMPLEMENTATION MI:0809 BiFC split fluorescent protein assay
YEAST_TWO_HYBRID MI:0018 Y2H screening in yeast
MAMMALIAN_TWO_HYBRID MI:2413 Two-hybrid in mammalian cells

Slots

Name Description
interaction_detection_method Methods used to detect molecular interactions

Identifier and Mapping Information

Annotations

property value
standard PSI-MI 2.5
maintainer HUPO Proteomics Standards Initiative

Schema Source

  • from schema: https://w3id.org/linkml/valuesets

LinkML Source

name: InteractionDetectionMethod
conforms_to: HUPO-PSI
annotations:
  standard:
    tag: standard
    value: PSI-MI 2.5
  maintainer:
    tag: maintainer
    value: HUPO Proteomics Standards Initiative
description: Methods used to detect molecular interactions
title: Interaction Detection Method
from_schema: https://w3id.org/linkml/valuesets
source: http://purl.obolibrary.org/obo/mi.owl
rank: 1000
permissible_values:
  TWO_HYBRID:
    text: TWO_HYBRID
    description: Classical two-hybrid system using transcriptional activity
    meaning: MI:0018
  COIMMUNOPRECIPITATION:
    text: COIMMUNOPRECIPITATION
    description: Using antibody to capture bait and its ligands
    meaning: MI:0019
  PULL_DOWN:
    text: PULL_DOWN
    description: Affinity capture using immobilized bait
    meaning: MI:0096
  TANDEM_AFFINITY_PURIFICATION:
    text: TANDEM_AFFINITY_PURIFICATION
    description: TAP tagging for protein complex purification
    meaning: MI:0676
  FLUORESCENCE_RESONANCE_ENERGY_TRANSFER:
    text: FLUORESCENCE_RESONANCE_ENERGY_TRANSFER
    description: FRET for detecting proximity between molecules
    meaning: MI:0055
    aliases:
    - fluorescent resonance energy transfer
  SURFACE_PLASMON_RESONANCE:
    text: SURFACE_PLASMON_RESONANCE
    description: SPR for real-time binding analysis
    meaning: MI:0107
  CROSS_LINKING:
    text: CROSS_LINKING
    description: Chemical cross-linking of interacting proteins
    meaning: MI:0030
    aliases:
    - cross-linking study
  X_RAY_CRYSTALLOGRAPHY:
    text: X_RAY_CRYSTALLOGRAPHY
    description: Crystal structure determination
    meaning: MI:0114
  NMR:
    text: NMR
    description: Nuclear magnetic resonance spectroscopy
    meaning: MI:0077
    aliases:
    - nuclear magnetic resonance
  ELECTRON_MICROSCOPY:
    text: ELECTRON_MICROSCOPY
    description: EM for structural determination
    meaning: MI:0040
  MASS_SPECTROMETRY:
    text: MASS_SPECTROMETRY
    description: MS-based interaction detection
    meaning: MI:0943
    aliases:
    - detection by mass spectrometry
  PROXIMITY_LIGATION_ASSAY:
    text: PROXIMITY_LIGATION_ASSAY
    description: PLA for detecting protein proximity
    meaning: MI:0813
  BIMOLECULAR_FLUORESCENCE_COMPLEMENTATION:
    text: BIMOLECULAR_FLUORESCENCE_COMPLEMENTATION
    description: BiFC split fluorescent protein assay
    meaning: MI:0809
  YEAST_TWO_HYBRID:
    text: YEAST_TWO_HYBRID
    description: Y2H screening in yeast
    meaning: MI:0018
    aliases:
    - two hybrid
  MAMMALIAN_TWO_HYBRID:
    text: MAMMALIAN_TWO_HYBRID
    description: Two-hybrid in mammalian cells
    meaning: MI:2413
    aliases:
    - mammalian membrane two hybrid