Enum: PCROperationTypeEnum

Types of PCR operations and techniques

Permissible Values

Value	Meaning	Description
STANDARD_PCR	None	Standard endpoint PCR amplification
QUANTITATIVE_PCR	None	Real-time quantitative PCR
REVERSE_TRANSCRIPTION_PCR	None	PCR with reverse transcription step for RNA amplification
RT_QPCR	None	Real-time quantitative RT-PCR
MULTIPLEX_PCR	None	PCR amplifying multiple targets simultaneously
NESTED_PCR	None	Two-stage PCR for increased specificity
TOUCHDOWN_PCR	None	PCR with decreasing annealing temperature
HOT_START_PCR	None	PCR with heat-activated polymerase
LONG_RANGE_PCR	None	PCR optimized for amplifying long DNA fragments
COLONY_PCR	None	PCR directly from bacterial colonies
HIGH_FIDELITY_PCR	None	PCR using proofreading polymerases
DIGITAL_PCR	None	Absolute quantification PCR using partitioning

Slots

Name	Description
pcr_operation_type	Type of PCR operation

Identifier and Mapping Information

Schema Source

from schema: https://w3id.org/linkml/valuesets

LinkML Source

name: PCROperationTypeEnum
description: Types of PCR operations and techniques
from_schema: https://w3id.org/linkml/valuesets
rank: 1000
permissible_values:
  STANDARD_PCR:
    text: STANDARD_PCR
    description: Standard endpoint PCR amplification
    title: standard PCR
  QUANTITATIVE_PCR:
    text: QUANTITATIVE_PCR
    description: Real-time quantitative PCR
    annotations:
      abbreviation:
        tag: abbreviation
        value: qPCR
    title: quantitative PCR
  REVERSE_TRANSCRIPTION_PCR:
    text: REVERSE_TRANSCRIPTION_PCR
    description: PCR with reverse transcription step for RNA amplification
    annotations:
      abbreviation:
        tag: abbreviation
        value: RT-PCR
    title: reverse transcription PCR
  RT_QPCR:
    text: RT_QPCR
    description: Real-time quantitative RT-PCR
    title: RT-qPCR
  MULTIPLEX_PCR:
    text: MULTIPLEX_PCR
    description: PCR amplifying multiple targets simultaneously
    title: multiplex PCR
  NESTED_PCR:
    text: NESTED_PCR
    description: Two-stage PCR for increased specificity
    title: nested PCR
  TOUCHDOWN_PCR:
    text: TOUCHDOWN_PCR
    description: PCR with decreasing annealing temperature
    title: touchdown PCR
  HOT_START_PCR:
    text: HOT_START_PCR
    description: PCR with heat-activated polymerase
    title: hot start PCR
  LONG_RANGE_PCR:
    text: LONG_RANGE_PCR
    description: PCR optimized for amplifying long DNA fragments
    title: long range PCR
  COLONY_PCR:
    text: COLONY_PCR
    description: PCR directly from bacterial colonies
    title: colony PCR
  HIGH_FIDELITY_PCR:
    text: HIGH_FIDELITY_PCR
    description: PCR using proofreading polymerases
    title: high fidelity PCR
  DIGITAL_PCR:
    text: DIGITAL_PCR
    description: Absolute quantification PCR using partitioning
    title: digital PCR