Enum: ProteinEngineeringAssayEnum

Assays for protein engineering including binding kinetics, biophysical characterization, and display-based selection methods.

URI: valuesets:ProteinEngineeringAssayEnum

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Permissible Values

Value	Meaning	Description	Companion Enum Readout	Companion Enum Target Protein
SURFACE_PLASMON_RESONANCE	BAO:0000054	Real-time label-free measurement of biomolecular binding kinetics	DetectionModeEnum	Protein
BIOLAYER_INTERFEROMETRY	BAO:0000066	Label-free measurement of biomolecular interactions via interference patterns	DetectionModeEnum	Protein
THERMAL_SHIFT_ASSAY	BAO:0010261	Measurement of protein thermal stability via fluorescent dye binding	DetectionModeEnum	Protein
CIRCULAR_DICHROISM	BAO:0000161	Assessment of protein secondary and tertiary structure using polarized light	DetectionModeEnum	Protein
PHAGE_DISPLAY		Selection of peptides or proteins displayed on bacteriophage surface		Protein
YEAST_DISPLAY		Selection of proteins displayed on yeast cell surface		Protein
DIRECTED_EVOLUTION_SCREEN		High-throughput screening of mutant protein libraries for improved function	DetectionModeEnum	Protein

Slots

Name	Description
protein_engineering_assay	Protein engineering assay type

Identifier and Mapping Information

Schema Source

from schema: https://w3id.org/valuesets

LinkML Source

name: ProteinEngineeringAssayEnum
instantiates:
- valuesets_meta:ValueSetEnumDefinition
description: Assays for protein engineering including binding kinetics, biophysical
  characterization, and display-based selection methods.
title: Protein Engineering Assay Types
from_schema: https://w3id.org/valuesets
contributors:
- orcid:0000-0002-6601-2165
- https://github.com/anthropics/claude-code
status: DRAFT
rank: 1000
permissible_values:
  SURFACE_PLASMON_RESONANCE:
    text: SURFACE_PLASMON_RESONANCE
    description: Real-time label-free measurement of biomolecular binding kinetics
    meaning: BAO:0000054
    annotations:
      companion_enum_target_protein:
        tag: companion_enum_target_protein
        value: Protein
      companion_enum_readout:
        tag: companion_enum_readout
        value: DetectionModeEnum
    aliases:
    - SPR
  BIOLAYER_INTERFEROMETRY:
    text: BIOLAYER_INTERFEROMETRY
    description: Label-free measurement of biomolecular interactions via interference
      patterns
    meaning: BAO:0000066
    annotations:
      companion_enum_target_protein:
        tag: companion_enum_target_protein
        value: Protein
      companion_enum_readout:
        tag: companion_enum_readout
        value: DetectionModeEnum
    aliases:
    - BLI
    - bio layer interferometry
  THERMAL_SHIFT_ASSAY:
    text: THERMAL_SHIFT_ASSAY
    description: Measurement of protein thermal stability via fluorescent dye binding
    meaning: BAO:0010261
    annotations:
      companion_enum_target_protein:
        tag: companion_enum_target_protein
        value: Protein
      companion_enum_readout:
        tag: companion_enum_readout
        value: DetectionModeEnum
    aliases:
    - differential scanning fluorimetry
    - DSF
  CIRCULAR_DICHROISM:
    text: CIRCULAR_DICHROISM
    description: Assessment of protein secondary and tertiary structure using polarized
      light
    meaning: BAO:0000161
    annotations:
      companion_enum_target_protein:
        tag: companion_enum_target_protein
        value: Protein
      companion_enum_readout:
        tag: companion_enum_readout
        value: DetectionModeEnum
    aliases:
    - CD
  PHAGE_DISPLAY:
    text: PHAGE_DISPLAY
    description: Selection of peptides or proteins displayed on bacteriophage surface
    annotations:
      companion_enum_target_protein:
        tag: companion_enum_target_protein
        value: Protein
  YEAST_DISPLAY:
    text: YEAST_DISPLAY
    description: Selection of proteins displayed on yeast cell surface
    annotations:
      companion_enum_target_protein:
        tag: companion_enum_target_protein
        value: Protein
  DIRECTED_EVOLUTION_SCREEN:
    text: DIRECTED_EVOLUTION_SCREEN
    description: High-throughput screening of mutant protein libraries for improved
      function
    annotations:
      companion_enum_target_protein:
        tag: companion_enum_target_protein
        value: Protein
      companion_enum_readout:
        tag: companion_enum_readout
        value: DetectionModeEnum