Enum: ThermalCyclingStepEnum

Steps in thermal cycling protocols

Permissible Values

Value	Meaning	Description
INITIAL_DENATURATION	None	Initial high-temperature step to denature template DNA
DENATURATION	None	High-temperature step to separate DNA strands
ANNEALING	None	Cooling step to allow primer binding
EXTENSION	None	Temperature-optimized step for polymerase activity
FINAL_EXTENSION	None	Extended final extension step
HOLD	None	Temperature hold step
MELT_CURVE	None	Gradual temperature increase for melt curve analysis
GRADIENT	None	Temperature gradient across block

Identifier and Mapping Information

Schema Source

from schema: https://w3id.org/linkml/valuesets

LinkML Source

name: ThermalCyclingStepEnum
description: Steps in thermal cycling protocols
from_schema: https://w3id.org/linkml/valuesets
rank: 1000
permissible_values:
  INITIAL_DENATURATION:
    text: INITIAL_DENATURATION
    description: Initial high-temperature step to denature template DNA
    title: initial denaturation
  DENATURATION:
    text: DENATURATION
    description: High-temperature step to separate DNA strands
    title: denaturation
  ANNEALING:
    text: ANNEALING
    description: Cooling step to allow primer binding
    title: annealing
  EXTENSION:
    text: EXTENSION
    description: Temperature-optimized step for polymerase activity
    title: extension
  FINAL_EXTENSION:
    text: FINAL_EXTENSION
    description: Extended final extension step
    title: final extension
  HOLD:
    text: HOLD
    description: Temperature hold step
    title: hold
  MELT_CURVE:
    text: MELT_CURVE
    description: Gradual temperature increase for melt curve analysis
    title: melt curve
  GRADIENT:
    text: GRADIENT
    description: Temperature gradient across block
    title: gradient