Enum: VitrificationMethod

Methods for sample vitrification

Permissible Values

Value	Meaning	Description
PLUNGE_FREEZING	None	Plunge freezing in liquid ethane
HIGH_PRESSURE_FREEZING	None	High pressure freezing
SLAM_FREEZING	None	Slam freezing against metal block
SPRAY_FREEZING	None	Spray freezing into liquid nitrogen

Slots

Name	Description
vitrification_method	Methods for sample vitrification

Identifier and Mapping Information

Schema Source

from schema: https://w3id.org/linkml/valuesets

LinkML Source

name: VitrificationMethod
description: Methods for sample vitrification
from_schema: https://w3id.org/linkml/valuesets
rank: 1000
permissible_values:
  PLUNGE_FREEZING:
    text: PLUNGE_FREEZING
    description: Plunge freezing in liquid ethane
    annotations:
      temperature:
        tag: temperature
        value: -180°C ethane
      equipment:
        tag: equipment
        value: Vitrobot, Leica GP
  HIGH_PRESSURE_FREEZING:
    text: HIGH_PRESSURE_FREEZING
    description: High pressure freezing
    annotations:
      pressure:
        tag: pressure
        value: 2100 bar typical
      advantages:
        tag: advantages
        value: thick samples, no ice crystals
  SLAM_FREEZING:
    text: SLAM_FREEZING
    description: Slam freezing against metal block
    annotations:
      cooling_rate:
        tag: cooling_rate
        value: 10,000 K/s
  SPRAY_FREEZING:
    text: SPRAY_FREEZING
    description: Spray freezing into liquid nitrogen
    annotations:
      applications:
        tag: applications
        value: large samples, tissues